pTOPO-HqERV(C1-20)
(Plasmid #164090)

• Purpose: transfection of Hq-ERV with light-inducible promoter
• Depositing Lab: Maik Hintze
• Publication: Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s00335-020-09854-0. doi: 10.1007/s00335-020-09854-0.

Backbone

• Vector backbone: pCR-XL-2-TOPO
• Backbone manufacturer: Invitrogen/ThermoFisher
• Backbone size w/o insert (bp): 3923
• Vector type: eukaryotic expression vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hq-ERV
• Species: M. musculus (mouse)
• Insert Size (bp): 8491
• Mutation: 5'-LTR replaced with light-regulatable C1-20 promoter
• Promoter: light-activatable C1-20

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (destroyed during cloning)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pTOPO-HqERV(C1-20).gb
• A portion of this plasmid was derived from a plasmid made by: Laura Motta-Mena/Kevin Gardner at UTSW Dallas. The C1-20 insert was derived from the pcDNA-C120-empty plasmid cited in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944926/.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The C1-20 insert was derived from the pcDNA-C120-empty plasmid (Motta-Mena and Gardner) and introduced into pTOPO-HqERV (Addgene plasmid #164088) to generate the current plasmid, pTOPO-HqERV(C1-20).