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pTOPO-HqERV(del5'-LTR)
(Plasmid #164089)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-XL-2-TOPO
  • Backbone manufacturer
    Invitrogen/ThermoFisher
  • Backbone size w/o insert (bp) 3929
  • Vector type
    eukaryotic expression vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hq-ERV
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    8283
  • Mutation
    deletion of 5'-LTR
  • Promoter none (5'-LTR deleted)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTOPO-HqERV(del5'-LTR) was a gift from Maik Hintze (Addgene plasmid # 164089 ; http://n2t.net/addgene:164089 ; RRID:Addgene_164089)

  • For your References section:

    Alopecia in Harlequin mutant mice is associated with reduced AIF protein levels and expression of retroviral elements. Hintze M, Griesing S, Michels M, Blanck B, Wischhof L, Hartmann D, Bano D, Franz T. Mamm Genome. 2020 Dec 26. pii: 10.1007/s00335-020-09854-0. doi: 10.1007/s00335-020-09854-0. 10.1007/s00335-020-09854-0 PubMed 33367954
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