pTOPO-HqERV(del5'-LTR)
(Plasmid
#164089)
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Purposetransfection of Hq-ERV without 5'-LTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-XL-2-TOPO
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Backbone manufacturerInvitrogen/ThermoFisher
- Backbone size w/o insert (bp) 3929
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Vector typeeukaryotic expression vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHq-ERV
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SpeciesM. musculus (mouse)
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Insert Size (bp)8283
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Mutationdeletion of 5'-LTR
- Promoter none (5'-LTR deleted)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTOPO-HqERV(del5'-LTR) was a gift from Maik Hintze (Addgene plasmid # 164089 ; http://n2t.net/addgene:164089 ; RRID:Addgene_164089) -
For your References section:
Alopecia in Harlequin mutant mice is associated with reduced AIF protein levels and expression of retroviral elements. Hintze M, Griesing S, Michels M, Blanck B, Wischhof L, Hartmann D, Bano D, Franz T. Mamm Genome. 2020 Dec 26. pii: 10.1007/s00335-020-09854-0. doi: 10.1007/s00335-020-09854-0. 10.1007/s00335-020-09854-0 PubMed 33367954