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Addgene

pGGG_Sr1644-1Sh
(Plasmid #164087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGolden Green Gate
  • Backbone manufacturer
    Hayta et al (2019) https://doi.org/10.1186/s13007-019-0503-z
  • Backbone size w/o insert (bp) 5722
  • Total vector size (bp) 16973
  • Vector type
    Bacterial Expression, Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Please note plasmid requires the helper plasmid, pSOUP (Addgene ID # 165419) for replication in Agrobacterium.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pGGG_Sr-1644-1Sh
  • Species
    Aegilops sharonensis
  • Insert Size (bp)
    11252
  • Promoter Native promoter
  • Tag / Fusion Protein
    • none

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGG_Sr1644-1Sh was a gift from Brande Wulff (Addgene plasmid # 164087 ; http://n2t.net/addgene:164087 ; RRID:Addgene_164087)

  • For your References section:

    Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62. Yu G, Matny O, Champouret N, Steuernagel B, Moscou MJ, Hernandez-Pinzon I, Green P, Hayta S, Smedley M, Harwood W, Kangara N, Yue Y, Gardener C, Banfield MJ, Olivera PD, Welchin C, Simmons J, Millet E, Minz-Dub A, Ronen M, Avni R, Sharon A, Patpour M, Justesen AF, Jayakodi M, Himmelbach A, Stein N, Wu S, Poland J, Ens J, Pozniak C, Karafiatova M, Molnar I, Dolezel J, Ward ER, Reuber TL, Jones JDG, Mascher M, Steffenson BJ, Wulff BBH. Nat Commun. 2022 Mar 25;13(1):1607. doi: 10.1038/s41467-022-29132-8. 10.1038/s41467-022-29132-8 PubMed 35338132
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