pCMV-MtPylRS (human-opti)
(Plasmid
#164080)
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PurposeTo express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 5988
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMtPylRS-478(human-opti)
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SpeciesMethanosarcina thermophila
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Insert Size (bp)1434
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-forward
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The insert gene-MtPylRS was retrieved from the uniprot database, where its ID is Q1L6A3. The genetic codon of the insert gene has been human optimized
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-MtPylRS (human-opti) was a gift from Tao Liu (Addgene plasmid # 164080 ; http://n2t.net/addgene:164080 ; RRID:Addgene_164080) -
For your References section:
Thermophilic Pyrrolysyl-tRNA Synthetase Mutants for Enhanced Mammalian Genetic Code Expansion. Hu L, Qin X, Huang Y, Cao W, Wang C, Wang Y, Ling X, Chen H, Wu D, Lin Y, Liu T. ACS Synth Biol. 2020 Oct 16;9(10):2723-2736. doi: 10.1021/acssynbio.0c00257. Epub 2020 Sep 23. 10.1021/acssynbio.0c00257 PubMed 32931698