pcDNA-NBid-PhoCl1-CBid
(Plasmid
#164050)
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PurposeOptogenetic manipulation of cell apoptosis using PhoCl1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1+
- Backbone size w/o insert (bp) 5350
- Total vector size (bp) 6709
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNBid-PhoCl1-CBid
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SpeciesSynthetic
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Insert Size (bp)1359
- Promoter CMV promotor
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Tag
/ Fusion Protein
- kozak sequence (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CCTCGACTG TGCCTTCTA
- 3′ sequencing primer CG CAAATGGGCG GTAGGCGTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.12.10.419556v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-NBid-PhoCl1-CBid was a gift from Robert Campbell (Addgene plasmid # 164050 ; http://n2t.net/addgene:164050 ; RRID:Addgene_164050) -
For your References section:
Photocleavable proteins that undergo fast and efficient dissociation. Lu X, Wen Y, Zhang S, Zhang W, Chen Y, Shen Y, Lemieux MJ, Campbell RE. Chem Sci. 2021 May 31;12(28):9658-9672. doi: 10.1039/d1sc01059j. eCollection 2021 Jul 21. 10.1039/d1sc01059j PubMed 34349937