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BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP
(Plasmid #164038)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-14xGFP11
  • Species
    Synthetic
  • Mutation
    D10A and H840A in Cas9
  • Promoter dSV40

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP was a gift from Baohui Chen (Addgene plasmid # 164038 ; http://n2t.net/addgene:164038 ; RRID:Addgene_164038)

  • For your References section:

    Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag. Chen B, Zou W, Xu H, Liang Y, Huang B. Nat Commun. 2018 Nov 29;9(1):5065. doi: 10.1038/s41467-018-07498-y. 10.1038/s41467-018-07498-y PubMed 30498221
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