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ASC-CASP1 Octamer
(Plasmid #164032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDB.His.MBP
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CASP1, ASC
  • Species
    H. sapiens (human)
  • Mutation
    W169G (ASC), G20K (CASP1)
  • Entrez Gene
    CASP1 (a.k.a. ICE, IL1BC, P45)
  • Entrez Gene
    PYCARD (a.k.a. ASC, CARD5, TMS, TMS-1, TMS1)
  • Tag / Fusion Protein
    • His6-MBP-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ASC-CASP1 Octamer was a gift from Hao Wu (Addgene plasmid # 164032 ; http://n2t.net/addgene:164032 ; RRID:Addgene_164032)

  • For your References section:

    Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes. Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H. Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y. 10.1038/s41467-020-20320-y PubMed 33420033
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