pShuttle-CMV
(Plasmid #16403)

• Purpose: (Empty Backbone) Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
• Depositing Lab: Bert Vogelstein
• Publication: He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.

Backbone

• Vector backbone: pShuttle-CMV
• Backbone size (bp): 7452
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• GenBank ID: AF334399

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • AdEasy Protocol
• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Max insert size = 6.6kb with pAdEasy1 and 9.3kb with pAdEasy2.

See AdEasy Protocol linked in the supplemental documents section above for more information.

NOTE: There are discrepancies between the Addgene quality control sequence and the assembled sequence from the Vogelstein lab. These differences are not known to affect function.

Information for Cloning Grade DNA (Catalog # 16403-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here