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pDB.His.MBP.3C CARD8 CARD A452C R495E
(Plasmid #164028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 164028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDB.His.MBP.3C
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CARD8
  • Species
    H. sapiens (human)
  • Mutation
    A452C, R495E
  • Entrez Gene
    CARD8 (a.k.a. CARDINAL, DACAR, DAKAR, NDPP, NDPP1, TUCAN)
  • Tag / Fusion Protein
    • His6-MBP-3C (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDB.His.MBP.3C CARD8 CARD A452C R495E was a gift from Hao Wu (Addgene plasmid # 164028 ; http://n2t.net/addgene:164028 ; RRID:Addgene_164028)

  • For your References section:

    Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes. Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H. Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y. 10.1038/s41467-020-20320-y PubMed 33420033
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