pShuttle
(Plasmid #16402)

• Purpose: (Empty Backbone) Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
• Depositing Lab: Bert Vogelstein
• Publication: He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.

Backbone

• Vector backbone: pShuttle
• Backbone size (bp): 6736
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• GenBank ID: AF334399

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • AdEasy Protocol
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.

See attached protocol and http://www.coloncancer.org/adeasy.htm

for more information.