pAdEasy-2
(Plasmid #16401)

• Purpose: (Empty Backbone) Adenoviral vector for use in AdEasy System, for use with large inserts.
• Depositing Lab: Bert Vogelstein
• Publication: He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.

Backbone

• Vector backbone: pAdEasy-2
• Backbone size (bp): 30767
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• GenBank ID: AY370909

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • AdEasy Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy-2.

Plasmids recombined in pAdEasy-2 should be packaged in 911E4 cells to make virus.

See attached protocol and http://www.coloncancer.org/adeasy.htm

for more information.

AdEasy is a registered trademark of the Johns Hopkins University.