pAdEasy-1
(Plasmid #16400)

• Purpose: (Empty Backbone) Adenoviral vector for use in AdEasy System.
• Depositing Lab: Bert Vogelstein
• Publication: He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.

Backbone

• Vector backbone: pAdEasy and reg-1
• Backbone size (bp): 33414
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• GenBank ID: AY370909

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • AdEasy Protocol
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy-1.

Plasmids recombined in pAdEasy-1 should be packaged in either 293 or 911 cells to make virus.

See attached protocol for more information.

AdEasy is a registered trademark of the Johns Hopkins University.

NOTE: Addgene was unable to obtain high quality sequence for this plasmid, which may due to its size and repeat regions. Please take this into consideration when conducting your own quality control.

Information for Cloning Grade DNA (Catalog # 16400-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here