pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R
(Plasmid
#163990)
-
PurposeMammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1 LIC 6A
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNLRP1
-
SpeciesH. sapiens (human)
-
MutationE1461R
-
Entrez GeneNLRP1 (a.k.a. AIADK, CARD7, CIDED, CLR17.1, DEFCAP, DEFCAP-L/S, JRRP, MSPC, NAC, NALP1, PP1044, SLEV1, VAMAS1)
-
Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R was a gift from Hao Wu (Addgene plasmid # 163990 ; http://n2t.net/addgene:163990 ; RRID:Addgene_163990) -
For your References section:
Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes. Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H. Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y. 10.1038/s41467-020-20320-y PubMed 33420033
