AdEasier-1 cells (strain)
(Bacterial strain #16399)

• Depositing Lab: Bert Vogelstein
• Publication: He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.

Backbone

• Vector backbone: n/a

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Streptomycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AdEasier-1 bacterial cells
• Alt name: BJAdEasy Cells
• Alt name: AdEasier Cells
• Species: E. coli
• GenBank ID: AY370909

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • AdEasy Protocol
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system.

AdEasier is a trademark of the Johns Hopkins University.