BJ5183 cells
(Bacterial strain #16398)

• Depositing Lab: Bert Vogelstein
• Publication: He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.

Backbone

• Vector backbone: n/a

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BJ5183 bacterial cells
• Species: E. coli

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • AdEasy Protocol
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a bacterial strain - not a plasmid. Select with 30 ug/mL streptomycin.

Electrocompetent BJ5183 cells are used to carry out the recombination between the shuttle vector (pShuttle, pShuttle-CMV, pAdTrack, or pAdTrack-CMV) and the adenoviral vector (pAdEasy1 or pAdEasy2).

See attached protocol and http://www.coloncancer.org/adeasy.htm

for more information.