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Addgene

pLEX307 ASC
(Plasmid #163977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX307
  • Backbone manufacturer
    Addgene #41392
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASC
  • Species
    H. sapiens (human)
  • Entrez Gene
    PYCARD (a.k.a. ASC, CARD5, TMS, TMS-1, TMS1)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307 ASC was a gift from Daniel Bachovchin (Addgene plasmid # 163977 ; http://n2t.net/addgene:163977 ; RRID:Addgene_163977)

  • For your References section:

    Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes. Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H. Nat Commun. 2021 Jan 8;12(1):189. doi: 10.1038/s41467-020-20320-y. 10.1038/s41467-020-20320-y PubMed 33420033
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