pTXB1-TnY
(Plasmid
#163975)
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PurposeIPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTXB1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTnY
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Insert Size (bp)1377
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Tag
/ Fusion Protein
- Mxe intein - Chitin-binding domain
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene verified sequence shows a different overall vector size compared to the author's map.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTXB1-TnY was a gift from Peter Smibert (Addgene plasmid # 163975 ; http://n2t.net/addgene:163975 ; RRID:Addgene_163975) -
For your References section:
Profiling the genetic determinants of chromatin accessibility with scalable single-cell CRISPR screens. Liscovitch-Brauer N, Montalbano A, Deng J, Mendez-Mancilla A, Wessels HH, Moss NG, Kung CY, Sookdeo A, Guo X, Geller E, Jaini S, Smibert P, Sanjana NE. Nat Biotechnol. 2021 Apr 29. pii: 10.1038/s41587-021-00902-x. doi: 10.1038/s41587-021-00902-x. 10.1038/s41587-021-00902-x PubMed 33927415
Map uploaded by the depositor.
