pRS423-SgH
(Plasmid
#163972)
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Purpose(Empty Backbone) Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163972 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS423
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Vector typeYeast Expression, Synthetic Biology
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS423-SgH was a gift from Jiazhang Lian (Addgene plasmid # 163972 ; http://n2t.net/addgene:163972 ; RRID:Addgene_163972) -
For your References section:
A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae. Dong C, Jiang L, Xu S, Huang L, Cai J, Lian J, Xu Z. ACS Synth Biol. 2020 Sep 18;9(9):2252-2257. doi: 10.1021/acssynbio.0c00218. Epub 2020 Aug 28. 10.1021/acssynbio.0c00218 PubMed 32841560