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Addgene

pRS415-Cas9-VPR
(Plasmid #163971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PRS415
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-VPR
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS415-Cas9-VPR was a gift from Jiazhang Lian (Addgene plasmid # 163971 ; http://n2t.net/addgene:163971 ; RRID:Addgene_163971)

  • For your References section:

    A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae. Dong C, Jiang L, Xu S, Huang L, Cai J, Lian J, Xu Z. ACS Synth Biol. 2020 Sep 18;9(9):2252-2257. doi: 10.1021/acssynbio.0c00218. Epub 2020 Aug 28. 10.1021/acssynbio.0c00218 PubMed 32841560
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