Mouse Metabolism Library
(Pooled Library #163966)

• Purpose:

  This lentiviral CRISPR library targets mouse metabolic genes with ~6 guide RNAs (sgRNAs) per gene.

• Vector Backbone:

  lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

• Depositing Labs: Alec Kimmelman
• Publication: Biancur et al Cell Metab. 2020 Oct 28. pii: S1550-4131(20)30551-9. doi: 10.1016/j.cmet.2020.10.018.

Library Details

• Species: Mouse
• Genes targeted: 2,918
• gRNAs: 18,343
• Controls: 1,000 intergenic controls
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (PDF, 176 KB)
  • PCR protocol for Illumina sequencing preparation (PDF, 143 KB)
• Depositor Data:
  • Table of targeted genes and gRNA sequences (XLSX, 1.6 MB)
  • Distribution of NGS Reads (Histogram) (PDF, 124 KB)
  • Distribution of NGS Reads (Table) (CSV, 235 KB)
  • Merged normalized read counts (TXT, 13 MB)
• Depositor software:
  • PoolQ-2.2.0 (ZIP, 17 MB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing lab has shared a GitHub repository with code for screening analysis.