Skip to main content
Addgene

pED9x (dCas9-KRAB-mCherry)
(Plasmid #163956)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pED9x
  • Backbone size (bp) 11549
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter U6 promoter for crRNA expression and EFS promoter for mCherry expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pED9x (dCas9-KRAB-mCherry) was a gift from Junwei Shi (Addgene plasmid # 163956 ; http://n2t.net/addgene:163956 ; RRID:Addgene_163956)

  • For your References section:

    Human epigenetic and transcriptional T cell differentiation atlas for identifying functional T cell-specific enhancers. Giles JR, Manne S, Freilich E, Oldridge DA, Baxter AE, George S, Chen Z, Huang H, Chilukuri L, Carberry M, Giles L, Weng NP, Young RM, June CH, Schuchter LM, Amaravadi RK, Xu X, Karakousis GC, Mitchell TC, Huang AC, Shi J, Wherry EJ. Immunity. 2022 Mar 8;55(3):557-574.e7. doi: 10.1016/j.immuni.2022.02.004. 10.1016/j.immuni.2022.02.004 PubMed 35263570
Related items:
Commonly requested with: