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Rock Lab Mycobacterium smegmatis CRISPRi Library
(Pooled Library #163955)

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  • Purpose

    This CRISPR repression library inhibits expression of open reading frames (ORFs) and non-coding RNAs in M. smegmatis (RLC11).

    The anhydrotetracycline (ATc)-inducible CRISPRi library was constructed in an L5-integrating backbone. It can be used in competitive growth experiments to reveal gene vulnerability.

  • Vector Backbone

    pIRL117 (Plasmid #163635) - expresses dCas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 163955 RLC11 gRNA Library 1 $640 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    M. smegmatis
  • Genes targeted
    6,642
  • gRNAs
    176,286
  • Controls
    7,421

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Code and methods for vulnerability studies with these libraries can be found in the Rock Lab's GitHub repository (Link opens in a new window).

See the STAR methods in Bosch et al. 2021 (Link opens in a new window) for PCR and sequencing protocols.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mycobacterium smegmatis CRISPRi Library RLC11 was a gift from Jeremy Rock (Addgene #163955)

  • For your References section:

    Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosis. Bosch B, DeJesus MA, Poulton NC, Zhang W, Engelhart CA, Zaveri A, Lavalette S, Ruecker N, Trujillo C, Wallach JB, Li S, Ehrt S, Chait BT, Schnappinger D, Rock JM Cell. 2021 Jul 16. pii: S0092-8674(21)00824-2. doi: 10.1016/j.cell.2021.06.033. PubMed 34297925 (Link opens in a new window)
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