5UTR_Mppetb_short_chlo
(Plasmid
#163933)
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PurposeL0 part - 5'UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepUAP4
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMarchantia polymorpha petB 5'UTR
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SpeciesOther
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5UTR_Mppetb_short_chlo was a gift from Jim Haseloff (Addgene plasmid # 163933 ; http://n2t.net/addgene:163933 ; RRID:Addgene_163933) -
For your References section:
Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts. Frangedakis E, Guzman-Chavez F, Rebmann M, Markel K, Yu Y, Perraki A, Tse SW, Liu Y, Rever J, Sauret-Gueto S, Goffinet B, Schneider H, Haseloff J. ACS Synth Biol. 2021 Jul 16;10(7):1651-1666. doi: 10.1021/acssynbio.0c00637. Epub 2021 Jun 7. 10.1021/acssynbio.0c00637 PubMed 34097383