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pUASTLOTattB_mCherry::Nrv1::vhhGFP4
(Plasmid #163926)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163926 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASTLOTattB
  • Backbone manufacturer
    Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014)
  • Backbone size w/o insert (bp) 8900
  • Total vector size (bp) 11000
  • Vector type
    Insect Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
  • Alt name
    GrabFP-B intracellular
  • Species
    D. melanogaster (fly)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To generate a basolateral GrabFP construct that exposes the nanobody to the cytosol we started with the GrabFP-BExt plasmid. The tagBFP sequence was replaced by the vhhGFP4 sequence and the original vhhGFP4 sequence was exchanged with an mCherry coding sequence.

pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).

vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.

mCherry: Clonetech

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTLOTattB_mCherry::Nrv1::vhhGFP4 was a gift from Markus Affolter (Addgene plasmid # 163926 ; http://n2t.net/addgene:163926 ; RRID:Addgene_163926)

  • For your References section:

    A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila. Harmansa S, Alborelli I, Bieli D, Caussinus E, Affolter M. Elife. 2017 Apr 11;6. doi: 10.7554/eLife.22549. 10.7554/eLife.22549 PubMed 28395731
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