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Addgene

pGL4-23-NegCtrl
(Plasmid #163904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163904 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.23-GW
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Species
    Firefly
  • Promoter minimal

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A silent region of chromatin was inserted upstream of the Firefly luciferase gene as a negative control for luciferase reporter assays.

Please visit https://www.biorxiv.org/content/10.1101/2021.12.23.474040v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4-23-NegCtrl was a gift from Alexander Ruthenburg (Addgene plasmid # 163904 ; http://n2t.net/addgene:163904 ; RRID:Addgene_163904)

  • For your References section:

    The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine transcription. Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, Šćepanović J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz IP, Ruthenburg AJ. bioRxiv 2021.12.23.474040 10.1101/2021.12.23.474040
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