Skip to main content
Addgene

pJJF152_Phsp_Cas9
(Plasmid #163862)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 3702
  • Total vector size (bp) 7899
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9, C. elegans codon optimized with intron and SV40_NLS
  • Species
    Synthetic
  • Insert Size (bp)
    4197
  • Promoter Phsp-16.48

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This plasmid uses the codon optimized Cas9 from Friedland et al. 2013 (PMID 23817069) which is distributed by Addgene as plasmid #46168.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid uses the codon optimized Cas9 from Friedland et al. 2013 (PMID 23817069) which is distributed by Addgene as plasmid #46168.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJJF152_Phsp_Cas9 was a gift from Nikolaus Rajewsky (Addgene plasmid # 163862 ; http://n2t.net/addgene:163862 ; RRID:Addgene_163862)

  • For your References section:

    Parallel genetics of regulatory sequences using scalable genome editing in vivo. Froehlich JJ, Uyar B, Herzog M, Theil K, Glazar P, Akalin A, Rajewsky N. Cell Rep. 2021 Apr 13;35(2):108988. doi: 10.1016/j.celrep.2021.108988. 10.1016/j.celrep.2021.108988 PubMed 33852857
Related items: