pT3TS-TurboID-EGFP
(Plasmid
#163846)
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PurposeIn vitro transcription of TurboID-EGFP. Parton lab clone JRS
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163846 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT3TS-Dest_R1-R3 (Addgene 140878)
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Vector typeIn vitro transcription of RNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTurboID
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SpeciesSynthetic
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT3TS-TurboID-EGFP was a gift from Rob Parton (Addgene plasmid # 163846 ; http://n2t.net/addgene:163846 ; RRID:Addgene_163846) -
For your References section:
In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish. Xiong Z, Lo HP, McMahon KA, Martel N, Jones A, Hill MM, Parton RG, Hall TE. Elife. 2021 Feb 16;10. pii: 64631. doi: 10.7554/eLife.64631. 10.7554/eLife.64631 PubMed 33591275