pSB-CMV-MCS-Puro dr_cPLA2-mKate2
(Plasmid #163844)

• Purpose: Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.
• Depositing Lab: Philipp Niethammer
• Publication: Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi: 10.1016/j.cell.2016.04.016.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 8685
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cytosolic phospholipase a2
• Alt name: cPLA2
• Alt name: pla2g4aa
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2224
• GenBank ID: 30554
• Entrez Gene: pla2g4aa (a.k.a. PLA2G4A, cpla2, pla2g4)
• Promoter: CMV
• Tag / Fusion Protein:
  • mKate2 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGHR

Resource Information

• Supplemental Documents:
  • pSB-CMV-MCS-Puro dr_cPLA2-mKate2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry