pB2H12*
(Plasmid
#163838)
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PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12 (Y64A)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSC101
- Backbone size w/o insert (bp) 3280
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSpecR
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Alt namec-Src kinase (251-536), CDC37, PTPN12 (1-306, Y64A)
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Alt namecI_SH2*;RpoZ_MidT
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)5593
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MutationPTPN12 Y64A
- Promoter Pro1;ProD;pLacZ-Opt
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byVarious addgene plasmids
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB2H12* was a gift from Jerome Fox (Addgene plasmid # 163838 ; http://n2t.net/addgene:163838 ; RRID:Addgene_163838)
