pSYC-437
(Plasmid #163829)

• Purpose: Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.
• Depositing Lab: Seok-Yong Choi
• Publication: Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi: 10.1038/s41467-020-15248-2.

Backbone

• Vector backbone: pDestTol2pA2
• Backbone size w/o insert (bp): 4464
• Total vector size (bp): 11016
• Vector type: MultiSite Gateway destination vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Foxj1a Promoter
• Species: D. rerio (zebrafish)
• Insert Size (bp): 5195

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: AGC AGG AAA CGT GAG CAG AG
• 3′ sequencing primer: ATG ATG ATG AGA ACC CAT GG

Gene/Insert 2

• Gene/Insert name: GCaMP6s
• Species: Synthetic
• Insert Size (bp): 1357

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GAA CTG GGA TTG GAC CTT TG
• 3′ sequencing primer: ATG TAA AAC GAC GGC CAG TG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The foxj1a promoter plasmid was provided by Dr. Brian Ciruna, Toronto, Canada. [Science. 2016 Jun 10;352(6291):1341-4. doi: 10.1126/science.aaf6419.]

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry