ShaCas9
(Plasmid
#163794)
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Purpose(Empty Backbone) Expresses ShaCas9, and cloning backbone for sgRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163794 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2 ITR vector
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ShaCas9 was a gift from Yongming Wang (Addgene plasmid # 163794 ; http://n2t.net/addgene:163794 ; RRID:Addgene_163794) -
For your References section:
Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity. Hu Z, Zhang C, Wang S, Gao S, Wei J, Li M, Hou L, Mao H, Wei Y, Qi T, Liu H, Liu D, Lan F, Lu D, Wang H, Li J, Wang Y. Nucleic Acids Res. 2021 Mar 15. pii: 6171296. doi: 10.1093/nar/gkab148. 10.1093/nar/gkab148 PubMed 33721016