BlatCas9
(Plasmid
#163792)
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Purpose(Empty Backbone) The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2 ITR vector
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Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BlatCas9 was a gift from Yongming Wang (Addgene plasmid # 163792 ; http://n2t.net/addgene:163792 ; RRID:Addgene_163792) -
For your References section:
Characterization of Brevibacillus laterosporus Cas9 (BlatCas9) for Mammalian Genome Editing. Gao N, Zhang C, Hu Z, Li M, Wei J, Wang Y, Liu H. Front Cell Dev Biol. 2020 Oct 19;8:583164. doi: 10.3389/fcell.2020.583164. eCollection 2020. 10.3389/fcell.2020.583164 PubMed 33195228