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pRRL-MND-hCard11 R35Q-T2A-GFP
(Plasmid #163774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PRRL
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARD11
  • Species
    H. sapiens (human)
  • Mutation
    R35Q
  • Entrez Gene
    CARD11 (a.k.a. BENTA, BIMP3, CARMA1, IMD11, IMD11A, PPBL)
  • Promoter MND

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC identified a G928V difference in the hCard11 insert that is of unknown functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRL-MND-hCard11 R35Q-T2A-GFP was a gift from Richard James (Addgene plasmid # 163774 ; http://n2t.net/addgene:163774 ; RRID:Addgene_163774)

  • For your References section:

    Multiplexed Functional Assessment of Genetic Variants in CARD11. Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper MA, Starita LM, Rawlings DJ, Snow AL, James RG. Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 10.1016/j.ajhg.2020.10.015. 10.1016/j.ajhg.2020.10.015 PubMed 33202260
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