pRRL-MND-hCard11 delta e4-T2A-GFP
(Plasmid
#163771)
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PurposeExpression of CARD domain variant with a deletion of exon 4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePRRL
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCARD11
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SpeciesH. sapiens (human)
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MutationDel exon 4
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Entrez GeneCARD11 (a.k.a. BENTA, BIMP3, CARMA1, IMD11, IMD11A, PPBL)
- Promoter MND
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRL-MND-hCard11 delta e4-T2A-GFP was a gift from Richard James (Addgene plasmid # 163771 ; http://n2t.net/addgene:163771 ; RRID:Addgene_163771) -
For your References section:
Multiplexed Functional Assessment of Genetic Variants in CARD11. Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper MA, Starita LM, Rawlings DJ, Snow AL, James RG. Am J Hum Genet. 2020 Nov 12. pii: S0002-9297(20)30373-6. doi: 10.1016/j.ajhg.2020.10.015. 10.1016/j.ajhg.2020.10.015 PubMed 33202260