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pTU1-A-SP44-sfGFP
(Plasmid #163757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTU1-A
  • Backbone manufacturer
    Freemont lab
  • Backbone size w/o insert (bp) 2326
  • Total vector size (bp) 3043
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • GenBank ID
    ASL68970.1
  • Promoter SP44
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer VF2
  • 3′ sequencing primer VR
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTU1-A-SP44-sfGFP was a gift from Paul Freemont & Simon Moore (Addgene plasmid # 163757 ; http://n2t.net/addgene:163757 ; RRID:Addgene_163757)

  • For your References section:

    A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology. Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre C, de Los Santos EL, Freemont PS. ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 10.1021/acssynbio.0c00581. Epub 2021 Jan 26. 10.1021/acssynbio.0c00581 PubMed 33497199
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