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Addgene

TET-pLKO-neo NXPH4-sh2
(Plasmid #163745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO-Tet-On (Tet-pLKO-Neo) Addgene # 21916
  • Backbone manufacturer
    Wiederschain et al., 2009 Cell Cycle 8:3, 498-504
  • Backbone size w/o insert (bp) 9084
  • Total vector size (bp) 9143
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NXPH4
  • gRNA/shRNA sequence
    CATCTGTATCTTCGTCTCTTT
  • Species
    H. sapiens (human)
  • Entrez Gene
    NXPH4 (a.k.a. NPH4)
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pLKO-seq: ggcagggatattcaccattatcgtttcaga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TET-pLKO-neo NXPH4-sh2 was a gift from Roland Friedel (Addgene plasmid # 163745 ; http://n2t.net/addgene:163745 ; RRID:Addgene_163745)
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