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Addgene

pJH773_pTech_PlldR-HHRz-supP(opal)
(Plasmid #163732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hhrz-supP
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH773_pTech_PlldR-HHRz-supP(opal) was a gift from Matthew Bennett (Addgene plasmid # 163732 ; http://n2t.net/addgene:163732 ; RRID:Addgene_163732)

  • For your References section:

    A suppressor tRNA-mediated feedforward loop eliminates leaky gene expression in bacteria. Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR. Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179. 10.1093/nar/gkaa1179 PubMed 33290521
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