pJH26_pBad_AraC_Para-sfGFP.1TAG
(Plasmid
#163721)
-
PurposeArabinose-inducible expression of green fluorescent protein encoding one amber codon
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 163721 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBR322
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namearaC gfp.1TAG
-
SpeciesSynthetic
-
Mutationgfp.N39TAG
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJH26_pBad_AraC_Para-sfGFP.1TAG was a gift from Matthew Bennett (Addgene plasmid # 163721 ; http://n2t.net/addgene:163721 ; RRID:Addgene_163721) -
For your References section:
A suppressor tRNA-mediated feedforward loop eliminates leaky gene expression in bacteria. Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR. Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179. 10.1093/nar/gkaa1179 PubMed 33290521