EC0_2_VP64
(Plasmid
#163714)
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PurposePlasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMoClo-YTK
- Total vector size (bp) 1849
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Vector typeSynthetic Biology ; Moclo vector level 0 (type 4a)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameVP64 + SV40 NLS
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Alt nameTetrameric repeat of the minimal activation domain of herpes simplex virus VP16 + SV40 nuclear localization signal
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Insert Size (bp)174
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Golden Gate Assembly (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EC0_2_VP64 was a gift from Yvonne Nygård (Addgene plasmid # 163714 ; http://n2t.net/addgene:163714 ; RRID:Addgene_163714) -
For your References section:
A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12. Camara E, Lenitz I, Nygard Y. Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w. 10.1038/s41598-020-71648-w PubMed 32884066
Map uploaded by the depositor.
