EC2_10_dCas9_VPR_HC_sgRNA
(Plasmid #163712)

• Purpose: Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains
• Depositing Lab: Yvonne Nygård
• Publication: Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w.

Backbone

• Vector backbone: MoClo-YTK
• Total vector size (bp): 11420
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: dCas9
• Alt name: Nuclease-deficient Cas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 4101
• Mutation: Point mutations D10A and H840A
• Promoter: ScTEF1

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Golden Gate Assembly

Gene/Insert 2

• Gene/Insert name: VPR
• Alt name: Fusion of the activation domains VP64, p65 and Rta
• Species: Synthetic
• Insert Size (bp): 1596

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry