EC2_1_dCas9_sgRNA
(Plasmid
#163710)
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PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassette
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMoClo-YTK
- Total vector size (bp) 9354
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Vector typeYeast Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedCas9
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Alt nameNuclease-deficient Cas9
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SpeciesStreptococcus pyogenes
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Insert Size (bp)4101
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MutationPoint mutations D10A and H840A
- Promoter ScTEF1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Golden Gate Assembly (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EC2_1_dCas9_sgRNA was a gift from Yvonne Nygård (Addgene plasmid # 163710 ; http://n2t.net/addgene:163710 ; RRID:Addgene_163710) -
For your References section:
A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12. Camara E, Lenitz I, Nygard Y. Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w. 10.1038/s41598-020-71648-w PubMed 32884066
Map uploaded by the depositor.
