EC2_2_dCas9_VP64_sgRNA
(Plasmid #163707)

• Purpose: Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain
• Depositing Lab: Yvonne Nygård
• Publication: Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w.

Backbone

• Vector backbone: MoClo-YTK
• Total vector size (bp): 9532
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: dCas9
• Alt name: Nuclease-deficient Cas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 3933
• Mutation: Point mutations D10A and H840A
• Promoter: ScTEF1

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Golden Gate Assembly

Gene/Insert 2

• Gene/Insert name: VP64+SV40 NLS
• Alt name: Tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 + SV40 nuclear localization signal
• Species: Herpes simplex virus
• Insert Size (bp): 174

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Golden Gate Assembly

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry