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pGGG-AH-VRT-A2
(Plasmid #163703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGoldenGreenGate (pGGG)
  • Backbone size w/o insert (bp) 2831
  • Total vector size (bp) 14901
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
  • Species
    Synthetic
  • Insert Size (bp)
    2892
  • Promoter Rice - Os Actin promoter

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Golden Gate BpiI (destroyed during cloning)
  • 3′ cloning site Golden Gate BpiI (destroyed during cloning)
  • 5′ sequencing primer LB Forward 5’-GTGGTGTAAACAAATTGACGC-3’
  • 3′ sequencing primer Actin P Forward 5’-CGTATCAAAGTACCGACAAAAACA-3’
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Triticum polonicum VRT-A2 genomic sequence (Native promoter:: genomic sequence::3'UTR) + Nos Terminator
  • Species
    Triticum polonicum
  • Insert Size (bp)
    8916
  • Promoter Triticum polonicum VRT-A2

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Golden Gate BsaI (destroyed during cloning)
  • 3′ cloning site Golden Gate BsaI (destroyed during cloning)
  • 5′ sequencing primer Actin P Forward 5’-CGTATCAAAGTACCGACAAAAACA-3’
  • 3′ sequencing primer RB-R1 Reverse 5’-CCCGCCAATATATCCTGTCA-3’
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGG-AH-VRT-A2 was a gift from Cristobal Uauy (Addgene plasmid # 163703 ; http://n2t.net/addgene:163703 ; RRID:Addgene_163703)

  • For your References section:

    Ectopic expression of Triticum polonicum VRT-A2 underlies elongated glumes and grains in hexaploid wheat in a dosage-dependent manner. Adamski NM, Simmonds J, Brinton JF, Backhaus AE, Chen Y, Smedley M, Hayta S, Florio T, Crane P, Scott P, Pieri A, Hall O, Barclay JE, Clayton M, Doonan JH, Nibau C, Uauy C. Plant Cell. 2021 Aug 13;33(7):2296-2319. doi: 10.1093/plcell/koab119. 10.1093/plcell/koab119 PubMed 34009390
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