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pUASTLOTattB_eGFP::Dpp
(Plasmid #163702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163702 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASTLOTattB
  • Backbone manufacturer
    Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014)
  • Backbone size w/o insert (bp) 8900
  • Total vector size (bp) 4653
  • Vector type
    Insect Expression, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    decapentaplegic
  • Alt name
    BMP, TGF-β, shv, TGF-beta, shortvein
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2520
  • Entrez Gene
    dpp (a.k.a. Dmel_CG9885, BMP, Bmp, CG9885, DPP, DPP-C, Dm-DPP, DmDPP, Dmel\CG9885, Dpp, Dpp/TGFbeta, Hin-d, M(2)23AB, M(2)LS1, TGF-b, TGF-beta, TGFbeta, Tg, Tgfbeta, blk, ho, l(2)10638, l(2)22Fa, l(2)k17036, shv)
  • Tag / Fusion Protein
    • Tagged with eGFP (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The eGFP::DPP instert is based on the Dpp-GFP plasmid obtained from S. Cohen. Teleman, A. A. & Cohen, S. M. Dpp gradient formation in the Drosophila wing imaginal disc. Cell 103, 971–980 (2000).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For pUASTLOTattB_eGFP::Dpp, GFP was replaced by eGFP in the Dpp-GFP plasmid (obtained from S. Cohen). Then, eGFP::Dpp was inserted in the multiple cloning site of pUASTLOTattB vector by standard cloning procedures.

pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).

Original Dpp-GFP plasmid:
Teleman, A. A. & Cohen, S. M. Dpp gradient formation in the Drosophila wing
imaginal disc. Cell 103, 971–980 (2000).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTLOTattB_eGFP::Dpp was a gift from Markus Affolter (Addgene plasmid # 163702 ; http://n2t.net/addgene:163702 ; RRID:Addgene_163702)

  • For your References section:

    Dpp spreading is required for medial but not for lateral wing disc growth. Harmansa S, Hamaratoglu F, Affolter M, Caussinus E. Nature. 2015 Nov 19;527(7578):317-22. doi: 10.1038/nature15712. Epub 2015 Nov 9. 10.1038/nature15712 PubMed 26550827
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