pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
(Plasmid #163698)

• Purpose: AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex
• Depositing Lab: Gina Turrigiano
• Publication: Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. doi: 10.1016/j.neuron.2020.11.027. Epub 2020 Dec 16.

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Backbone

• Vector backbone: pAAV
• Total vector size (bp): 5608
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
• Alt name: CaMKII-CaMPARI2
• Species: Synthetic
• Insert Size (bp): 1446
• Promoter: CaMKII fragment
• Tags / Fusion Proteins:
  • FLAG-HA-myc (C terminal on insert)
  • NES_his (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: f.hsyn
• 3′ sequencing primer: r.WPRE

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Schreiter lab at Janelia Research Campus

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CaMKII promoter fragment cloned into https://www.addgene.org/101060/ to replace hSyn promoter