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PurposeExpresses c-terminal half of TurboID on surface of astrocytes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZac2.1
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGPI anchored TurboID
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZac2.1-GfaABC1D-TurboID(C )-HA-GPI was a gift from Scott Soderling (Addgene plasmid # 163697 ; http://n2t.net/addgene:163697 ; RRID:Addgene_163697) -
For your References section:
Chemico-genetic discovery of astrocytic control of inhibition in vivo. Takano T, Wallace JT, Baldwin KT, Purkey AM, Uezu A, Courtland JL, Soderblom EJ, Shimogori T, Maness PF, Eroglu C, Soderling SH. Nature. 2020 Dec;588(7837):296-302. doi: 10.1038/s41586-020-2926-0. Epub 2020 Nov 11. 10.1038/s41586-020-2926-0 PubMed 33177716