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Addgene

LoriT
(Plasmid #163691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    L4440
  • Backbone manufacturer
    Andrew Fire (Addgene plasmid # 1654)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    oriT
  • Species
    E.coli

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aacgccagcaacgcggcct
  • 3′ sequencing primer tcagctcactcaaaggcgg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LoriT was a gift from Baris Tursun (Addgene plasmid # 163691 ; http://n2t.net/addgene:163691 ; RRID:Addgene_163691)

  • For your References section:

    The CONJUDOR pipeline for multiplexed knockdown of gene pairs identifies RBBP-5 as a germ cell reprogramming barrier in C. elegans. Kazmierczak M, Farre I Diaz C, Ofenbauer A, Herzog S, Tursun B. Nucleic Acids Res. 2021 Feb 26;49(4):e22. doi: 10.1093/nar/gkaa1171. 10.1093/nar/gkaa1171 PubMed 33290523
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