pAAV-HDR-sphys-3XHA
(Plasmid
#163690)
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Purposeplasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAddgene 104589
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametriple HA tag insertion into endogenous mouse synaptophysin gene
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- triple HA tag (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-HDR-sphys-3XHA was a gift from Bernardo Sabatini (Addgene plasmid # 163690 ; http://n2t.net/addgene:163690 ; RRID:Addgene_163690) -
For your References section:
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain. Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL. Elife. 2020 Oct 12;9. pii: 59699. doi: 10.7554/eLife.59699. 10.7554/eLife.59699 PubMed 33043885