pcDNA3.1 Orai2
(Plasmid #16369)

• Depositing Lab: Anjana Rao
• Publication: Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-43.

Backbone

• Vector backbone: pcDNA3.1/myc-his
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Orai2
• Species: H. sapiens (human)
• GenBank ID: BC069270
• Entrez Gene: ORAI2 (a.k.a. C7orf19, CBCIP2, MEM142B, TMEM142B)
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry