pAAV hSynapsin SV-tag
(Plasmid #163685)

• Purpose: express SV-Tag construct in a Cre-independent manner
• Depositing Lab: Bernardo Sabatini
• Publication: Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 10.7554/eLife.59699.

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Backbone

• Vector backbone: AAV
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: synatophysin 9X HA tag T2A tdtomato
• Species: M. musculus (mouse)
• Promoter: synapsin
• Tag / Fusion Protein:
  • 9X HA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: gggggcacgggcgcgaccatctg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry